The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR items had been digested and cloned right into a pET21a+ vector working with NdeI and XhoI restriction enzymes. Some extent mutation was released by the https://traudlp988dpz0.wikiannouncing.com/user